Fluorescence studies on the interaction between pyridoxal phosphate enzymes.
نویسندگان
چکیده
منابع مشابه
Interaction of pyridoxal analogues with glutamate apodecarboxylase and aspartate apoaminotransferase.
The interaction of a variety of pyridoxal analogues with glutamate decarboxylase and aspartate aminotransferase has been studied. Pyridoxal phosphate N-oxide, N-methylpyridoxal phosphate, 3-0-methylpyridoxal phosphate, 4-de(formyl)-4-vinyl pyridoxal phosphate, and pyridoxal analogues which contain modified substituents in position 5 (CH2CHrOPOsHL, CH(CHB)OPOIH2, CHVPOBHL, and CHICH,COOH) are bo...
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The reaction of cycloserine with pyridoxal5-phosphate has been investigated by fluorescence spectroscopy. The substantial change in fluorescence intensity at 450 rnp that follows the reaction of cycloserine with pyridoxal5-phosphate was used to monitor the release of the cofactor from the active site of the enzyme glutamate aspartate transaminase. A kinetic analysis of the fluorescence measurem...
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Physical studies have been performed on a genetically fused enzyme and its two component wild type enzymes, Lhistidinol dehydrogenase and imidazolylacetol phosphate: Lglutamate aminotransferase. The circular dichroic activity of the peptide chromophore of the fused enzyme is approximately accounted for by a weighted average of the activities of the two wild type enzymes. All three proteins lose...
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on pyridoxine or pyridoxamine as a sole source of carbon and nitrogen (1, 2). This transaminase contains no pyridoxal phosphate, and hence provides a simpler system for mechanistic studies than do the more complex, pyridoxal phosphate-containing transaminases. The enzyme binds pyridoxamine and pyridoxal with almost equal avidity; the latter is bound in part by an azomethine Iinkage to the e-ami...
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Proteins belonging to the superfamily of pyridoxal 5'-phosphate-dependent enzymes are currently classified into three functional groups and five distinct structural fold types. The variation within this enzyme group creates an ideal system to investigate the relationships among amino acid sequences, folding pathways, and enzymatic functions. The number of known three-dimensional structures of p...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 249 17 شماره
صفحات -
تاریخ انتشار 1974